MH (Mueller-Hinton) agar is a standard microbiological growth medium, primarily used for
antibiotic susceptibility testing(AST), especially with the Kirby-Bauer disk diffusion method, because it's non-selective, non-differential, and allows for consistent antibiotic diffusion, supporting a wide range of bacteria like
Enterobacteriaceae,
Staphylococci, and
Pseudomonas. Key ingredients include beef extract, casein hydrolysate (providing nutrients), starch (absorbing toxins), and agar (solidifying agent), with controlled levels of calcium and magnesium for specific antibiotic activity
